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import imagej from pathlib import Path from PIL import Image import scyjava import logging # Enable debug logging for scyjava logging.basicConfig(level=logging.DEBUG) # Ensure JVM is started scyjava.start_jvm() # Attempt to import the Java class try: Accesses = scyjava.jimport("net.imglib2.img.basictypeaccess.Accesses") print("Successfully imported Accesses class") except Exception as e: print("Error importing Accesses class:", e) def load_leica_image_to_imagej(imagej, image_path): # Load image into ImageJ imagej.ui().show(image_path) imagej.ui().waitForUI() imagej.ui().getWindow().pack() imagej.ui().getWindow().toFront() def export_image_sequence(imagej, output_folder, image_name_prefix): # Iterate through all open images in ImageJ for idx, image_id in enumerate(imagej.ui().getImageIDs()): # Activate each image imagej.ui().getImagePlus().setID(image_id) imagej.ui().getImagePlus().setSlice(1) # Get image data image_title = imagej.ui().getImagePlus().getTitle() img = imagej.ui().getImagePlus().getProcessor().getBufferedImage() # Save as JPEG with specified naming convention output_filename = f"{image_name_prefix}_{idx+1:05d}.jpg" output_path = output_folder / output_filename img_rgb = Image.fromarray(img) img_rgb.save(output_path) print(f"Saved {output_filename}") def main(): # Initialize ImageJ ij = imagej.init('net.imagej:imagej-legacy:0.37.1') # Path to the Leica image file leica_image_path = '/Volumes/Elements/WO153_pep22_16p25_240607.lif' # Load Leica image into ImageJ load_leica_image_to_imagej(ij, leica_image_path) # Export images as JPEG sequence output_folder = Path('/Volumes/Elements/results') image_name_prefix = 'WO153_pep22_16p25_240607' export_image_sequence(ij, output_folder, image_name_prefix) if __name__ == "__main__": main()
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