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flowchart LR A[Start] --> B{Obtain Coffee Beans} B --> C[Roast Coffee Beans] C --> D[Grind Coffee Beans] D --> E[Prepare Coffee Extract] E --> F[Dilute Coffee Extract] F --> G{Determine TPC & TFC} G --> H[Disk Diffusion Method] H --> I[Measure Zones of Inhibition] I --> J[Agar Dilution Method] J --> K{Determine MIC & MBC} K --> L[Statistical Analysis] L --> M[End] style A fill:#f9f,stroke:#333,stroke-width:2px style B fill:#f9f,stroke:#333,stroke-width:2px style C fill:#f9f,stroke:#333,stroke-width:2px style D fill:#f9f,stroke:#333,stroke-width:2px style E fill:#f9f,stroke:#333,stroke-width:2px style F fill:#f9f,stroke:#333,stroke-width:2px style G fill:#f9f,stroke:#333,stroke-width:2px style H fill:#f9f,stroke:#333,stroke-width:2px style I fill:#f9f,stroke:#333,stroke-width:2px style J fill:#f9f,stroke:#333,stroke-width:2px style K fill:#f9f,stroke:#333,stroke-width:2px style L fill:#f9f,stroke:#333,stroke-width:2px style M fill:#f9f,stroke:#333,stroke-width:2px
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