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flowchart LR
    A[Start] --> B{Obtain Coffee Beans}
    B --> C[Roast Coffee Beans]
    C --> D[Grind Coffee Beans]
    D --> E[Prepare Coffee Extract]
    E --> F[Dilute Coffee Extract]
    F --> G{Determine TPC & TFC}
    G --> H[Disk Diffusion Method]
    H --> I[Measure Zones of Inhibition]
    I --> J[Agar Dilution Method]
    J --> K{Determine MIC & MBC}
    K --> L[Statistical Analysis]
    L --> M[End]

    style A fill:#f9f,stroke:#333,stroke-width:2px
    style B fill:#f9f,stroke:#333,stroke-width:2px
    style C fill:#f9f,stroke:#333,stroke-width:2px
    style D fill:#f9f,stroke:#333,stroke-width:2px
    style E fill:#f9f,stroke:#333,stroke-width:2px
    style F fill:#f9f,stroke:#333,stroke-width:2px
    style G fill:#f9f,stroke:#333,stroke-width:2px
    style H fill:#f9f,stroke:#333,stroke-width:2px
    style I fill:#f9f,stroke:#333,stroke-width:2px
    style J fill:#f9f,stroke:#333,stroke-width:2px
    style K fill:#f9f,stroke:#333,stroke-width:2px
    style L fill:#f9f,stroke:#333,stroke-width:2px
    style M fill:#f9f,stroke:#333,stroke-width:2px
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