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graph LR A[Centrifuge culture, decant medium] --> B{Resuspend cells in saline} B --> C{Clean slide with soap, ethanol, flame} C --> D{Clip slide, add 500 µl cells} D --> E{Tilt slide 45°, let suspension run down} E --> F{Discard slide if beads form} E --> G{Air dry sample} G --> H{Draw rectangle with wax pencil} H --> I{Flood slide with Leifson's dye} I --> J{Incubate 7-15 minutes} J --> K{Rinse with tap water, air dry} K --> L{View under oil immersion objective}
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