Untitled

graph LR
A[Centrifuge culture, decant medium] --> B{Resuspend cells in saline}
B --> C{Clean slide with soap, ethanol, flame}
C --> D{Clip slide, add 500 µl cells}
D --> E{Tilt slide 45°, let suspension run down}
E --> F{Discard slide if beads form}
E --> G{Air dry sample}
G --> H{Draw rectangle with wax pencil}
H --> I{Flood slide with Leifson's dye}
I --> J{Incubate 7-15 minutes}
J --> K{Rinse with tap water, air dry}
K --> L{View under oil immersion objective}